Prime Pro 48 - Gerçek Zamanlı (Kantitatif) PCR Sistemi

Prime Pro 48 Real-time qPCR machine

The Prime Pro 48 real time qPCR system is a new, high specification, economically priced real time thermal cycler from Techne. Made in the UK, the Prime Pro 48 features the highest thermal block uniformity and fastest run time of any block-based qPCR system on the market.

The capacity of Prime Pro 48 accommodates a unique and economical 48-well qPCR plate. The well format mirrors a 384-well plate therefore allowing the use of 16-channel pipettes. The small qPCR plate cuts reagent costs in half whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity and greatly reduces run times.

Key benefits

• 40 cycles in 40 minutes before optimisation.
• 40 cycles in 15 minutes when optimised.
• Genotype in 4 minutes with over 99% accuracy.
• Set up a 4plex reaction in only 4 mouse clicks.

Technical Specification


High Resolution Melt Yes
Volume per well Validated for 5 to 20 ul
Detection sensitivity 1 copy
Temp uniformity +- 0.1°C
Temp accuracy +- 0.15°C @ 60°C and 95°C
Temperature range 30 to 100 degC
Average ramp rate 5.5 degC sec
Thermal system Hollow silver block peltier-based with conductive fluid
Block format 48-well block
Consumables 48-well custom plates and optical adhesive seals
Optical system Dual LED excitation (452 to 486 nm and 542 to 582 nm)
Camera CCD camera
Filters 4 emission filters (505 to 545nm 562 to 596nm 604 to 644nm 665 to 705nm)
Calibrated dyes SYBR FAM HEX ROX Cy5
Additional dyes All dyes within range compatible with no additional calibration
Passive reference dyes Use of ROX is supported but optional
Data collection Data always collected in all four filters for all wells
Plate setup Plate setup for data analysis can be altered after run completes
Melt curve Supports continuous data acquisition in a single filter to provide increased data point collection and reduced run times
PCR cycle time (unoptimised) 40 cycles in less than 40 minutes
PCR cycle time (FAST optimised) 40 cycles in less than 20 minutes
Dynamic range Greater than 9 logs
Calibration Not required
Installation Plug and play design
Precision Discriminates 5000 and 10000 template copies with 99 percent confidence
Warranty 1 year (parts and labour included)
Voltage 100 to 240 V.AC
Frequency 50 and 60 Hz
Nominal current draw 5A
Peak power 500W (typical power is 180W)
Software Multiple license software included at no additional cost
Chemistry All chemistries supported
Applications Absolute Quantification and Relative Quantification
Applications Allelic Discrimination and High Resolution Melt
Dimensions closed (WxDxH) 34.5cm x 31cm x 32cm (13.6 in x 12.2 in x 12.6 inches)
Dimensions open (WxDxH) 34.5cm x 31cm x 37cm (13.6 in x 12.2 in x 14.5 inches)
Weight 13.6 Kg (30 lb)

PCR/qPCR Cihazları